Western Blot of whole rat brain stem homogenate stained with 031421, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kD corresponds to Peripherin. A lower band at ~48kD is derived from the Peripherin molecule.

Western blot analysis of tissue and cell lysates using 031421 (1:10,000) in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kD corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.

Mixed neuron/glia cultures from newborn rat brain stained with 031421 (green) and chicken polyclonal antibody to phosphorylated NF-H (031399, red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain phosphorylated NF-H and not peripherin. The blue channel reveals DNA localized in nuclei of neurons and other cells.