Western Blot analysis using 043203 (1:500) Lane 1: Mouse lung tissue lysate Lane 2: Mouse liver tissue lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) goat anti-rabbit (1:10,000) Predicted band size: 25kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot analysis using 043203 (1:500-1000) Lane 1: Mouse kidney tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) goat anti-rabbit (1:10,000) Predicted band size: 25kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of paraffin-embedded mouse lung tissue using 043203 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9). Samples were incubated with 043203 (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemistry analysis of paraffin-embedded mouse brain tissue using 043203 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9). Samples were incubated with 043203 (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.