Proximity Ligation Analysis (PLA) of protein-protein interactions between RASGRP4 and RAP1A HeLa cells were stained with RASGRP4 rabbit purified polyclonal 1:1200 and RAP1A mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.