Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 351935.

Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using 351935 T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).

Immunohistochemical analysis of paraffin-embedded human tonsil using 351935.

Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using 351935. Controls were run on Jurkat cells (orange) and primary CD4+ T cells (red) using concentration-matched Rabbit mAb IgG Isotype Control. Anti-rabbit IgG (H+L), F(ab’)2 fragment was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml)