Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using 042220. This demonstrates that 042220 detected the SPECC1 protein (arrow).

Flow Cytometry analysis of HL-60 cells using 042220 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of U-251MG cells using 042220 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).