Affinity Purified Human SFRP1was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated prior to blocking. HRP Gt-a-Rabbit.
Western blot using Affinity Purified SFRP1 shows detection of a band~37kD (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of SFRP1 overnight at 4°C. Signal was detected using standard techniques.
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