Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution + ZF4 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution Lane 1: Zebrafish muscle lysate Lane 2: Zebrafish lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Z. skeletal muscle section using hsp90a. 1 Antibody (Center). hsp90a.1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from zebra fish heart tissue lysate, using (DANRE) hsp90a. 1 Antibody (Center). hsp90a.1 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.