Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using 032459 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 032459 for immunohistochemistry.

Confocal immunofluorescent analysis with Hela cell using 032459, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow Cytometric analysis of MCF-7 cells using 032459 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.