Western blot analysis is shown using C8454-04 to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat anti Rabbit IgG and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results.