Staining DKK1 in Human lung adenocarcinoma tissue sections by Immunohistochemistry (Immunohistochemistry (Paraffin) - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-DKK1 Antibody at 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 29kD Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DKK1 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 29kD Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-DKK1 Antibody at 1:4000 dilution + Hela whole cell lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 29kD Blocking/Dilution buffer: 5% NFDM/TBST.