Western Blot analysis using V2095-03A (1:2000) Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 85kD Blocking/Dilution buffer: 5% NFDM/TBST

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon carcinoma using V2095-03A followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of V2095-03A for immunohistochemistry.

Immunohistochemistry analysis in paraformaldehyde-fixed paraffin-embedded human lung tissue using V2095-03A. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer, pH 6. Samples were incubated with V2095-03A (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Indirect Flow Cytometric analysis of CEM cells using V2095-03A (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.