Confocal immunofluorescent analysis of 293T (left) or MCF7 cells (right), using Angiomotin Rabbit mAb (green). Actin filaments were labeled with 554 Phalloidin (red). Blue pseudocolor (fluorescent DNA dye).

Immunoprecipitation of Angiomotin from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit mAb Isotype Control, and lane 3 is Angiomotin Rabbit mAb. Western blot analysis was performed using Angiomotin Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Angiomotin p80 (hAngiomotin p80-Myc/DDK; +) protein, using Angiomotin Rabbit mAb (upper) or Myc-Tag Rabbit mAb (lower).