Western Blot analysis: Synthetic H1 (100ng) and recombinant H2 (500ng) relaxin were separated by SDS-PAGE and probed with R1425-02B (1:200).

Immunohistochemistry analysis of paraffin sections of left ventricular tissue from a patient with dilated cardiomyopathy. Image labeled B is tissue section incubated using R1425-02B (1:300) for 24h. DAB was used as chromogen; hematoxylin and eosin were used as counterstains. R1425-02B stains myocytes (not shown) and interstitial cells (asterisk) of the myocardium. Image labeled D is tissue section incubated with non-immune rabbit serum.