Western blot analysis is shown using Affinity Purified Swi6 to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of~43kD corresponding to S.pombe Swi6 protein.~35?g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 ?m nitrocellulose. The blot was incubated with a 1:1700 dilution at room temperature for 2h followed by detection using Goat-Rabbit IgG diluted 1:5000 for 45 min. Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.