Rabbit Anti-NFkB, p65, CT Antibody N2302-02 from United States Biological

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Rabbit Anti-NFkB, p65, CT Antibody

Immunohistochemistry of N2302-02. Tissue: lymphocytes and germinal center cells of the human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: N2302-02 at 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.
Rabbit Anti-NFkB, p65, CT Antibody

Western Blot of N2302-02. Lane 1: HeLa cell lysate. Lane 2: HeLa cell lysate. Load: 35ug per lane. Primary antibody: N2302-02 at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65kD, ~65kD for NFkb p65 Rel A. Other band(s): None.
Rabbit Anti-NFkB, p65, CT Antibody

Western Blot of N2302-02. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 10ug per lane. Primary antibody: N2302-02 at 1:500 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 65kD for NFkB.

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United States Biological for
Rabbit Anti-NFkB, p65, CT Antibody

United States Biological's Rabbit Anti-NFkB, p65, CT Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, Precipitation, ChIP, ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. The Rabbit Anti-NFkB, p65, CT Antibody was generated using RELA as the antigen and it reacts with Homo Sapiens, Human, and Mouse.

Rabbit Anti-NFkB, p65, CT Antibody

Rabbit Anti-NFkB, p65, CT Antibody

Rabbit Anti-NFkB, p65, CT Antibody

Rabbit Anti-NFkB, p65, CT Antibody from United States Biological

United States Biological

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Rabbit Anti-NFkB, p65, CT Antibody from United States Biological

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