Western Blot: all lanes using 034485 Lane 1: SH-SY5Y whole cell lysate Lane 2: mouse adrenal gland lysate Lane 3: rat adrenal gland lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) goat anti-rabbit. Predicted band size: 69kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis with A2058 cells using 034485 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with 034485 (1:25), followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1:200 (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 (red). The nuclear counter stain is DAPI (blue).