Western Blot analysis using 039528 (1:1000) Lane 1: A431 whole cell lysate Lane 2: Human heart whole tissue lysate Lane 3: Mouse kidney whole tissue lysate Lane 4: Rat kidney whole tissue lysate Lane Lane 5: HACAT whole cell lysate Lane 6: Mouse heart whole tissue lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit, (1:10,000). Predicted band size: 67kD Blocking/Dilution buffer: 5% NFDM/TBST.
Western Blot analysis using 039528 (1:1000) Lane 1: HACAT whole cell lysate Lane 2: A431 whole tissue lysate Lane 3: Mouse kidney whole tissue lysate Lane 4: Mouse heart whole tissue lysate Lane 5: Rat kidney whole cell lysate Lane Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit, (1:10,000). Predicted band size: 67kD Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry analysis in formalin fixed, paraffin embedded human stomach tissue using 039528 followed by peroxidase conjugated secondary antibody and DAB staining.This data demonstrates the use of 039528 for immunohistochemistry.
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