Immunofluorescence microscopy after staining of mouse carotid artery tissue with 168267 diluted 1:50. Tissue sections were prepared after cyrofixation. Reaction occurred at room temperature for 60' followed by washes and reaction with Rhodamine conjugated Gt-a-Rabbit IgG. Tissue was counterstained with bis-benzimide solution at 0.5mg/ml in PBS for 15 min at room temperature. Panel A) shows no staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1B staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-11B KO mouse.