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Mouse anti Pig SLA Class I antibody, clone JM1E3 (MCA2261GA) used for the evaluation of SLA Class I expression on bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Effect of pericardial fluid on pBM-MSCs phenotype. The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean ±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.

From: Citation: Blázquez R, Sánchez-Margallo FM, Crisístomo V, Báez C, Maestre J, García-Lindo M, et al. (2015)
Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis.
PLoS ONE 10(3): e0122377.
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