Fig 1: Wnt5a is involved in NLCs-induced survival and migration of CLL cells.A Cell viability analysis by trypan blue staining of CLL cells with or without NLCs treated by anti-Wnt5a or control mAb of irrelevant specificity for 24 h (n = 3). B Percentage of CLL cells migrating toward NLCs. CLL cells were treated by anti-Wnt5a or control mAb of irrelevant specificity for 4 h. Error bars denote standard deviation (n = 3). C Immunoblot analysis of phospho-ERK, total ERK, phospho-HS1, total HS1, phospho-p65, and total p65 in lysates of CLL cells with or without NLCs treated by anti-Wnt5a or control mAb of irrelevant specificity for 24 h. Dot figures represent ratios of densities (pERK/ERK, pHS1/HS1, and p-p65/p65).