Fig 1: Menin mediates tat-induced apoptosis in SH-SY5Y cellsMEN1 gene expression was knocked down using a specific sequence in SH-SY5Y cells stably transfected with pll3.7-MEN1-shRNA. MEN1 knockdown significantly quenches production of cleaved-caspase 3 and cleaved-caspase 8 induced by pRK5M-Tat-flag. Representative western blot A. and relative density of menin B. cleaved-caspase 8 C. and cleaved-caspase 3 D. TUNEL staining shows significantly increased apoptosis (green) in pRK5M-Tat-flag-transfected SH-SY5Y cells, and MEN1-knockdown reduced Tat-induced SY5Y cells apoptosis E-F. Data are plotted as mean ± SD (n = 3). *P < 0.05, **P < 0.01.