Fig 1: AID 2.1 enables minimal basal degradation, efficient target depletion, and faster dynamic recovery.a–c The western blots show basal and induced depletion of degron-tagged endogenous DNMT3B, U2AF2, and RBBP4 proteins in iPSCs expressing AID 2.0 (OsTIR1F74G) and AID 2.1 (OsTIR1F74G+S210A) after being treated with 1 μM 5-Phenyl-indole-3-acetic acid (5-Ph-IAA) for the indicated times. The bar plots show quantification of basal protein levels in both systems from three independent biological replicates. d The line plot shows Incucyte live cell imaging data indicating relative apoptosis rates in degron-tagged-RBBP4 iPSCs expressing OsTIR1F74G or OsTIR1F74G+S210A after DMSO control and 1 μM 5-Ph-IAA treatment. e The line plots show relative cell proliferation rates in degron-tagged-RBBP4 iPSCs expressing OsTIR1F74G or OsTIR1F74G+S210A and the wild-type parental cells without auxin treatment. f Western blots show the basal RBBP4 protein levels in degron-tagged-RBBP4 iPSCs expressing OsTIR1F74G or OsTIR1F74G+S210A. g The volcano plots show differential gene expression levels in degron-tagged RBBP4mAID/mAID iPSCs expressing AID 2.0 (left panel) or AID 2.1 (right panel) relative to the parental RBBP4WT/WT iPSCs. h Colony formation assay (quantified in bar plot) shows the remaining surviving iPSCs after ligand washout (after 6 h of 1 μM 5-Ph-IAA treatment) in degron-tagged-RBBP4 iPSCs expressing AID 2.0 or AID 2.1. i The Incucyte live cell imaging results show relative apoptosis rates in degron-tagged-RBBP4 iPSCs expressing AID 2.0 or AID 2.1 after auxin washout (after 3 h of 5-Ph-IAA treatment). Data and error bars in a–e and g–i indicate the means ± SD (n = 3 biological replicates in (a, b, d, e, g–i), n = 4 biological replicates in (c)). A two-sided unpaired t-test has been used for statistical analysis. Source data are provided as a Source Data file.