Fig 1: Spatial distribution of mesenchyme cells and chondroprogenitors in organoids(A) UMAP projections of snRNA-seq data and immunofluorescence of markers for mesenchyme cells (PTPRZ1, DDR1, TNC - day 87) and chondroprogenitors (TNC - day 28, PDGFRA, DCN) at different stages of differentiation: day 28 cells and organoids, and organoids at immature (day 87), and mature stages, where the morphology is that of elastic cartilage as in Figure 1C.(B) Expression of COL1A1 and collagen receptor DDR2 at day 28, day 87 (UMAP projections, left) and mature organoids (immunofluorescence, right). Scale bar = 50 μM.(C) Comparison of expression of mesenchyme marker PTPRZ1, and chondrocyte markers COL1A1 and DDR2 in individual cells at day 28 and 87. Blended colors (right) indicate co-expression in individual cells as yellow points.(D) Day 87 clusters and cell cycle assignments for individual cells. Cells and organoids at day 28 and 87 were differentiated from hESCs; mature organoids were differentiated from iPSCs (H20961, 120 days). N ≧ 2 for all IF results. iPS cells produced craniofacial cartilage in two experiments each from two different iPS lines.