Anti-GABA-A-R-Alpha5 Antibody 75-401 from Antibodies Inc.

Product Specs
ItemAnti-GABA-A-R-Alpha5 Antibody
CompanyAntibodies Inc.
Price $329.00Supplier Page View Company Product Page
Catalog Number75-401
Quantity100 µL
FormatLiquid, Purified by Protein A chromatography
ApplicationsWB, IHC, ICC, ELISA
Conjugate/TagUnconjugated
Concentration1 mg/mL
Antibody TypePrimary Antibody
ReactivityHuman, Mouse, and Rat
CloneN415/24
Gene NameGABRA5
HostMouse
Molecular Weight55 kDa
TargetGABA-A-R-Alpha5
IsotypeIgG1
ClonalityMonoclonal
Regulatory StatusThese antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans
ImmunogenFusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A-R-Alpha5 (accession number P31644) produced recombinantly in E. Coli
ValidationEach new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band
NCBI Gene AliasesDEE79, EIEE79

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Antibodies Inc.

P.O. Box 1560

Davis, CA 95617-1560

United States

Phone: (800) 824-8540

Email: orders@antibodiesinc.com

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Website: www.antibodiesinc.com/

(1) Glycerol enhances mitochondrial metabolism and inflammatory response in pro-inflammatory macrophagesEMBO ReportsApril 14, 2026Manami Tanaka, Takako Hishiki, Tomomi Matsuura, Masato Yasui, Shunsuke Chikuma, Mariko Hara-ChikumaChemical Corporation,Cat# 460-11892 TG-M test wako,WAKO,Cat# 464-89001 NEFA C-test Wako,WAKO,Cat# 279-75401 L-Type Wako LD・IF,WAKO,Cat＃460-88001 L-Type Wako AST・J2,WAKO,Cat＃464-87801 L-Type Wako ALT・J2,WAKO,Cat＃460-87901 L-Type

(2) ARHGEF3 coordinates adipocyte hypertrophy and differentiation through dual YAP-RhoA and PPARγ activationJournal of Advanced ResearchApril 9, 2025Sana Abdul Khaliq, Shi-Young Park, Syeda Maham, Yoonil Cho, Miseon Lee, Seungyoon Nam, Je Kyung Seong, Jie Chen, Cheol Soo Choi, Mee-Sup Yoonand plasma non-esterified fatty acid levels were quantified utilizing colorimetric assay kits (279-75401, Wako Pure Chemical Industries, Osaka, Japan). Plasma triglycerides, total cholesterol, low-density

(3) Extracellular vesicles contribute to the beneficial effects of exercise training in APP/PS1 miceiScienceFebruary 21, 2025Fuller OK, McLennan ED, Egan CL, Perera N, Terry LV, Pyun J, de Mendonca M, Telles GD, Smeuninx B, Burrows EL, et al.Dilution-Free™ Rapid Gold BCA Protein Assay,Thermo Fisher Scientific,Cat# A55860 NEFA C-test kit,Wako,Cat# 279-75401 Mouse Ultrasensitive Insulin ELISA kit,Alpco Diagnostics,Cat# 80-INSMSU-E01; RRID:AB_2792981 Amyloid

(4) Age-related TFEB downregulation in proximal tubules causes systemic metabolic disorders and occasional apolipoprotein A4-related amyloidosisJCI insightDecember 19, 2024Nakamura J, Yamamoto T, Takabatake Y, Namba-Hamano T, Takahashi A, Matsuda J, Minami S, Sakai S, Yonishi H, Maeda S, et al.Cholesterol E-test (Wako, 439–17501), the Triglyceride E-test (Wako, 432–40201), the NEFA C-test (Wako, 279–75401), the Phospholipid C-test (Wako, 433–36201), and Mouse/Rat Insulin ELISA kit (Morinaga, M1108). Urine

(5) A diet high in glucose and deficient in dietary fibre causes fat accumulation in the liver without weight gainBiochemistry and Biophysics ReportsDecember 1, 2024Sonoko Karino, Haruki Usuda, Shoma Kanda, Takayuki Okamoto, Tomomi Niibayashi, Takahisa Yano, Kohji Naora, Koichiro WadaCholesterol E test Wako, and NEFA C test Wako (Wako, Japan, cat.no. 432–40201, cat.no. 439–17501, Code 279–75401), respectively. Plasma AST and ALT levels were measured using Transferases C-II test Wako (Fujifilm

(6) Deletion of miPEP in adipocytes protects against obesity and insulin resistance by boosting muscle metabolismMolecular MetabolismAugust 1, 2024Alexis Diaz-Vegas, Kristen C. Cooke, Harry B. Cutler, Belinda Yau, Stewart W.C. Masson, Dylan Harney, Oliver K. Fuller, Meg Potter, Søren Madsen, Niamh R. Craw, et al.2000 ×g for 10 min at 4 °C. Plasma free fatty acids were measured using the NEFA C kit (Wako, Cat#279-75401). Plasma measurements before and after insulin injection utilised the 0 and 10-min plasma samples obtained

(7) The serum tenascin C level is a marker of metabolic disorder-related inflammation affecting pancreatic cancer prognosisScientific ReportsMay 26, 2024Katsuhiko Sato, Hayato Hikita, Minoru Shigekawa, Kazumasa Soma, Ryohei Yamauchi, Jihyun Sung, Seiya Kato, Yoichi Sasaki, Shinnosuke Kudo, Kenji Fukumoto, et al.Cambridge, UK) was used to measure the TNC concentration in human serum. An NEFA C test kit (Wako, #279-75401; Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) was used to measure serum NEFA concentrations

(8) Mitochondrial biogenesis in white adipose tissue mediated by JMJD1A-PGC-1 axis limits age-related metabolic diseaseiScienceApril 19, 2024Ryo Ito, Shiyu Xie, Myagmar Tumenjargal, Yuto Sugahara, Chaoran Yang, Hiroki Takahashi, Makoto Arai, Shin-Ichi Inoue, Aoi Uchida, Kenji Nakano, et al.assays,Critical commercial assays ,, Glucose C2 test kit,Wako,439-90901 NEFA C-test kit,Wako,279-75401 Autokit Total Ketone Bodies,Wako,415-73301 Triglyceride E-test kit,Wako,432-40201 Cholesterol E-test

(9) FGF21 and autophagy coordinately counteract kidney disease progression during aging and obesityAutophagyMarch 1, 2024Minami S, Sakai S, Yamamoto T, Takabatake Y, Namba-Hamano T, Takahashi A, Matsuda J, Yonishi H, Nakamura J, Maeda S, et al.Cholesterol E-test (Wako, 439–17501), the Triglyceride E-test (Wako, 432–40201), the NEFA C-test (Wako, 279–75401), the Phospholipid C-test (Wako, 433–36201), and the Mouse/Rat FGF21 Quantikine ELISA Kit (R&D Systems,

(10) Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional FactorsInternational Journal of Molecular SciencesMarch 30, 2023Ayumu Osaki, Kazuki Kagami, Yuki Ishinoda, Atsushi Sato, Toyokazu Kimura, Shunpei Horii, Kei Ito, Takumi Toya, Yasuo Ido, Takayuki Namba, et al.alanine aminotransferase (ALT) were measured using enzymatic assays (432-40201, 439-17501, 431-52501, 279-75401, and 431-30901, Wako Pure Chemical Industries, Osaka, Japan). An oral glucose tolerance test (OGTT)

(11) Pentraxin 3 is an adipose tissue‐related serum marker for pancreatic cancer cachexia predicting subsequent muscle mass and visceral fat lossCancer ScienceDecember 1, 2022Katsuhiko Sato, Hayato Hikita, Minoru Shigekawa, Seiya Kato, Yoichi Sasaki, Kazuma Shinkai, Makoto Fukuoka, Shinnosuke Kudo, Yu Sato, Kenji Fukumoto, et al.supernatant was also measured with a murine PTX3 ELISA kit (ab245713; Abcam). A NEFA C Test Wako (#279‐75401; Fujifilm Wako Pure Chemical Corporation) was used for NEFA measurement. Human serum was assayed with

(12) Acidic Activated Charcoal Prevents Obesity and Insulin Resistance in High-Fat Diet-Fed MiceFrontiers in NutritionMay 12, 2022Xuguang Zhang et al.(TGs) (#632-50991), total cholesterol (T-Chol) (#294-65801), non-esterified fatty acids (NEFAs) (#279-75401), phospholipids (PLs) (#433-36201), total bile acid (TBA) (#431-15001), and glucose (#298-65701) were

(13) Inducible Systemic Gcn1 Deletion in Mice Leads to Transient Body Weight Loss upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen StorageInternational journal of molecular sciencesMarch 16, 2022Liu J, Kasai S, Tatara Y, Yamazaki H, Mimura J, Mizuno S, Sugiyama F, Takahashi S, Sato T, Ozaki T, et al.were quantified using Glucagon ELISA kit (Mercodia, Uppsala, Sweden) and LabAssay NEFA kit (Wako, 279-75401), respectively, according to the manufacturer’s instruction. Lipoprotein profile of serum was performed

(14) Integrated stress response regulates GDF15 secretion from adipocytes, preferentially suppresses appetite for a high-fat diet and improves obesityiScienceDecember 17, 2021Miyake M, Zhang J, Yasue A, Hisanaga S, Tsugawa K, Sakaue H, Oyadomari M, Kiyonari H, Oyadomari S.Test,WAKO,Cat# 432-40201 Cholesterol E Test,WAKO,Cat# 439-17501 Non-esterified fatty acid E Test,WAKO,Cat# 279-75401 Mouse/Rat GDF-15 Quantikine ELISA Kit,R&D Systems,Cat# MGD150 Human GDF-15 Quantikine ELISA Kit,R&D Systems

(15) White Adipose Tissue Autophagy and Adipose-Liver Crosstalk Exacerbate Nonalcoholic Fatty Liver Disease in MiceCellular and Molecular Gastroenterology and HepatologyJuly 22, 2021Sadatsugu Sakane, Hayato Hikita, Kumiko Shirai, Yuta Myojin, Youichi Sasaki, Shinnosuke Kudo, Kenji Fukumoto, Naoki Mizutani, Yuki Tahata, Yuki Makino, et al.nonesterified fatty acid (NEFA) concentration was measured using a NEFA C Test Wako (catalog number 279-75401; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). The lipolysis ratio was calculated as the NEFA

(16) Ascorbic acid during the suckling period is required for proper DNA demethylation in the liverScientific ReportsDecember 4, 2020Kenichi Kawahori et al.UK). Biochemical assays Serum NEFA, TG, and T-Chol levels were measured using NEFA C-Test Wako (#279–75,401), TG E-Test Wako (#432–40,210), and T-Chol E-Test Wako (#439–17,501) kits (Wako Pure Chemical Industries,

(17) Mitochondrial uncoupler BAM15 reverses diet-induced obesity and insulin resistance in miceNature CommunicationsDecember 1, 2020Stephanie J. Alexopoulos et al.cholesterol (Thermo Scientific, TR13421), and fluorometric assay used to measure NEFA (Wako Chemical, 279-75401). All assays were conducted according to the manufacturer’s protocols. Results were normalised to mass

(18) The Effect of Heat Stress on Respiratory Alkalosis and Insulin Sensitivity in Cinnamon Supplemented PigsAnimals (Basel)April 15, 2020Jeremy J. Cottrell, John B. Furness, Udani A. Wijesiriwardana, Mitchell Ringuet, Fan Liu, Kristy DiGiacomo, Brian J. Leury, Iain J. J Clarke, Frank R. Dunshea(Data Cal VA, Thermo Scientific). Plasma NEFA concentrations were quantified using a kit (Cat no. 279-75401, Wako Diagnostics), as previously described [19]. The plasma insulin concentrations were measured in duplicate

(19) Ezetimibe suppresses development of liver tumors by inhibiting angiogenesis in mice fed a high‐fat dietCancer ScienceFebruary 1, 2019Kouichi Miura et al.439‐17501), Free Cholesterol E (435‐35801), Triglyceride E (432‐40201) and Non‐Esterified Fatty Acid C (279‐75401) kits, respectively, according to manufacturer (Wako Pure Chemical Industries, Osaka, Japan) instructions.

(20) Epigenetic modulation of Fgf21 in the perinatal mouse liver ameliorates diet-induced obesity in adulthoodYearbook of Paediatric EndocrinologySeptember 11, 2018Yuan X, Tsujimoto K, Hashimoto K, Kawahori K, Hanzawa N, Hamaguchi M, Seki T, Nawa M, Ehara T, Kitamura YQuantikine ELISA; R&D Systems). Serum NEFA, TG, and T-Chol levels were measured using NEFA C-Test Wako (#279-75401), TG E-Test Wako (#432-40210), and T-Chol E-Test Wako (#439-17501) kits (Wako Pure Chemical Industries,

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Anti-GABA-A-R-Alpha5 Antibody from Antibodies Inc.

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Antibodies Inc.

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Anti-GABA-A-R-Alpha5 Antibody from Antibodies Inc.

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