InVivoMAb anti-mouse FAP BE0374-R001mg from Bio X Cell

Product Specs
ItemInVivoMAb anti-mouse FAP
CompanyBio X Cell
Price $178.00Supplier Page View Company Product Page
Catalog NumberBE0374-R001mg
Quantity1 mg
ApplicationsFlow Cytometry, Immunoprecipitation, Immunoblot
ConcentrationLot-Specific
Conjugate/TagUnconjugated
FormatPBS, pH 7.0, Contains no stabilizers or preservatives, Liquid
Purity≥95% Determined by SDS-PAGE
ReactivityMouse
IsotypeIgG1, κ
Clone73.3
HostMouse
Antibody TypePrimary Antibody
ImmunogenMouse FAP-expressing 3T3 cells
NCBI Full Gene Namefibroblast activation protein
NCBI Gene AliasesSIMP
ClonalityMonoclonal
Research AreaCancer Biology, Immunology, Tumor Antigens
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze
Molecular Weight150 kDa
ModificationsNo
OriginUnited States

Specifications/Features

Quantities Available: 1mg, 5mg, 25mg, 50mg, 100mg, Additional Custom Sizing Available on bioxcell.com

Endotoxin: ≤1EU/mg (≤0.001EU/µg), Determined by LAL gel clotting assay

Questions and Answers

Can this antibody be used for flow cytometry applications in mouse models?

Yes, the InVivoMAb anti-mouse FAP monoclonal antibody has been reported for use in flow cytometry, making it suitable for analyzing mouse models.

What specific target does this antibody recognize, and why is that important in cancer research?

This antibody targets fibroblast activation protein (FAP), which is significant in cancer biology as FAP is often overexpressed in tumor-associated fibroblasts and is implicated in tumor progression and metastasis.

Is this antibody suitable for constructing chimeric antigen receptors, and what implications does that have for immunotherapy?

Yes, the InVivoMAb anti-mouse FAP has been noted for its application in chimeric antigen receptor construction, which is crucial for developing targeted immunotherapies that can enhance the immune response against tumors expressing FAP.

What are the purity and endotoxin levels of this antibody, and why are these factors important for experimental reliability?

The antibody has a purity greater than 95% and endotoxin levels of less than 2 EU/mg. High purity ensures minimal non-specific binding, while low endotoxin levels are critical to avoid unwanted immune activation in experimental settings, thus enhancing the reliability of results.

Bio X Cell

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Lebanon, NH 03766

United States

Phone: 1-866-787-3444

Email: customerservice@bioxcell.com

Company Profile

Website: bioxcell.com

(1) Loss of cells expressing fibroblast activation protein has variable effects in models of TGF-β and chronic bleomycin-induced fibrosisAmerican journal of physiology. Lung cellular and molecular physiologyAugust 1, 2019Kimura T, Monslow J, Klampatsa A, Leibowitz M, Sun J, Liousia M, Woodruff P, Moon E, Todd L, Puré E, et al.

(2) FAP Delineates Heterogeneous and Functionally Divergent Stromal Cells in Immune-Excluded Breast TumorsCancer immunology researchDecember 1, 2018Cremasco V, Astarita JL, Grauel AL, Keerthivasan S, MacIsaac K, Woodruff MC, Wu M, Spel L, Santoro S, Amoozgar Z, et al.

(3) Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in MiceThe Journal of biological chemistryApril 8, 2016Fan MH, Zhu Q, Li HH, Ra HJ, Majumdar S, Gulick DL, Jerome JA, Madsen DH, Christofidou-Solomidou M, Speicher DW, et al.

(4) Tumor-Promoting Desmoplasia Is Disrupted by Depleting FAP-Expressing Stromal CellsCancer researchJuly 15, 2015Lo A, Wang LS, Scholler J, Monslow J, Avery D, Newick K, O'Brien S, Evans RA, Bajor DJ, Clendenin C, et al.

(5) Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicityCancer immunology researchFebruary 1, 2014Wang LC, Lo A, Scholler J, Sun J, Majumdar RS, Kapoor V, Antzis M, Cotner CE, Johnson LA, Durham AC, et al.

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