CD45RO Antibody [SPM125] from Biorbyt

Application Notes: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.25-0.5 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.