Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/2,000 dilution. Lane 1: HepG2 cell lysate, 10 µg/Lane Lane 2: 293T cell lysate, 10 µg/Lane Lane 3: Rat testis tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.