Western blot analysis of SOX17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500476, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKOV-3 cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse testis tissue lysate Predicted band size: 44 kDa Observed band size: 55 kDa