Western blot analysis of P2Y12 on different lysates with Rabbit anti-P2Y12 antibody (HA721011) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.