Western blot analysis of IL-2RG on different lysates with Rabbit anti-IL-2RG antibody (HA500269) at 1/4,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: CTLL-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 55 kDa (Glycoprotein) Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500269) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.