Western blot analysis of GCSF Receptor on different lysates with rabbit anti-GCSF Receptor antibody (HA500282) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lane 3: SH-SY5Y cell lysate Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500282, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 92 kDa Observed band size: 92 kDa