Western blot analysis of TIM44 on different lysates with Rabbit anti-TIM44 antibody (HA721067) at 1/5,000 dilution. Lane 1: PC-3 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: LNCaP cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721067) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.