Western blot analysis of USP8 on different lysates with Rabbit anti-USP8 antibody (HA500362) at 1/500 dilution. Lane 1: A375 cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: 293T cell lysate (10 µg/Lane) Lane 5: MCF-7 cell lysate (10 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: SK-Br-3 cell lysate (10 µg/Lane) Lane 8: F9 cell lysate (10 µg/Lane) Lane 9: Rat lung tissue lysate (20 µg/Lane) Lane 10: Hela cell lysate (10 µg/Lane) Predicted band size: 128 kDa Observed band size: 128 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500362) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.