Western blot analysis of ITPK1 on different lysates with Rabbit anti-ITPK1 antibody (HA721039) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721039) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.