Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: 1 minute; 10 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody MYO19 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.