Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (HA721091) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.