Western blot analysis of RUNX3 on different lysates with Mouse anti-RUNX3 antibody (HA600058) at 1/5,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: Ramos cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44/46 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600058) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.