Western blot analysis of PSMD7 on different lysates with Rabbit anti-PSMD7 antibody (HA500354) at 1/500 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (16 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (12 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500354) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.