Western blot analysis of NSDHL on different lysates with Rabbit anti-NSDHL antibody (HA721088) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: HEK-293 cell lysate Lane 5: AGS cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 3 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.