Western blot analysis of MRPS15 on different lysates with Rabbit anti-MRPS15 antibody (HA720106) at 1/2,000 dilution. Lane 1: JAR cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 23 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720106) at 1/2,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.