Western blot analysis of Stathmin on different lysates with Rabbit anti-Stathmin antibody (HA721179) at 1/1,000 dilution. Lane 1: HeLa cell tissue lysate (20 µg/Lane) Lane 2: SH-SY5Y cell tissue lysate (20 µg/Lane) Lane 3: Jurkat cell tissue lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.