Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (HA500388) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: Human cerebellum tissue lysate (20 µg/Lane) Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 6: Rat cerebellum tissue lysate (20 µg/Lane) Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.