Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (HA721026) at 1/500 dilution. Lane 1: HCT116 cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse testis tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721026) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.