Western blot analysis of Nucleophosmin on different lysates with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: C2C12 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721206) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.