Western blot analysis of RBBP5 on different lysates with Rabbit anti-RBBP5 antibody (HA500395) at 1/1,000 dilution. Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate Lane 3: K562 cell lysate Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lane 8: N2A cell lysate Lane 9: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500395) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.