Coagulation Factor III/Tissue Factor Antibody AF3178 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCoagulation Factor III/Tissue Factor Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3178
NCBI Gene AliasesTF, Cf3, TFA, CD142, Cf-3
ApplicationsFACS,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,CyTOF-ready,ELISA Detection
Conjugate/TagUnconjugated
TargetCoagulation Factor III/Tissue Factor
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse,Transgenic Mouse
HostGoat
IsotypeIgG
NCBI Full Gene Namecoagulation factor III, tissue factor, coagulation factor III
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor, Ala29-Glu251, Accession # P20352
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Coagulation Factor III is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Nicotinamide Attenuates Complement and Coagulation Pathways and Resultant Renal FibrosisFASEB JDecember 15, 2025Saori Kin, Yuji Oe, Taku Obara, Emiko Sato, Nobuyuki Takahashi, Mariko Miyazaki, Tetsuhiro Tanakawere then cut into 3‐μm‐thick slices for immunohistochemistry. Goat anti‐mouse tissue factor (1:200, #AF3178, R&D Systems, Minneapolis, MN), goat anti‐mouse C3d antibody (1:2000, #AF2655, R&D Systems, Minneapolis,

(2) Nicotinamide Attenuates Complement and Coagulation Pathways and Resultant Renal FibrosisFASEB JDecember 1, 2025Saori Kin, Yuji Oe, Taku Obara, Emiko Sato, Nobuyuki Takahashi, Mariko Miyazaki, Tetsuhiro Tanakawere then cut into 3‐μm‐thick slices for immunohistochemistry. Goat anti‐mouse tissue factor (1:200, #AF3178, R&D Systems, Minneapolis, MN), goat anti‐mouse C3d antibody (1:2000, #AF2655, R&D Systems, Minneapolis,

(3) Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged miceMolecular MetabolismMay 1, 2025Shifeng Wang, Yuanxu Cui, Limei Wang, Chun Feng, Yifei Sun, Bangyun Huo, Honglu Jiang, Mingyu Zhao, Yingying Tu, Qiyue Wang, et al.and 0.1% Triton X-100 in PBS. Primary antibodies (UCP1, 1:100, CST, 72298; CD142, 1:100, R&D Systems, AF3178) were applied and incubated overnight at 4 °C. After washing with PBST, sections were incubated for

(4) Aging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogrammingElifeMay 22, 2024Corey D Holman, Alexander P Sakers, Ryan P Calhoun, Lan Cheng, Ethan C Fein, Christopher Jacobs, Linus Tsai, Evan D Rosen, Patrick Seale(Biolegend, 102528; 1:1000), and anti-mouse CD142 (Sino Biological, 50413-R001, 1:100; or R&D Systems, AF3178, 1:50). Anti-mouse CD142 antibodies were conjugated with Biotium Mix-n-Stain CF647 (Sigma, MX647S100).

(5) Western blot and immunohistochemical analysis of mouse tissue factorResearch and Practice in Thrombosis and HaemostasisNovember 2, 2023Nigel Mackman, Yohei Hisada, Jin-Ah ParkTF in cell lysates by western blotting. In contrast, a goat anti-mouse TF antibody from R&D Systems (AF3178) detected TF in the cell lysate by western blotting [4]. We also used AF3178 to demonstrate that TF

(6) Aging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogrammingbioRxiv : the preprint server for biologyMarch 23, 2023Holman CD, Sakers AP, Calhoun RP, Cheng L, Fein EC, Jacobs C, Tsai L, Rosen ED, Seale P.(Biolegend, 102528; 1:1000), and anti-mouse CD142 (Sino Biological, 50413-R001, 1:100; or R&D Systems, AF3178, 1:50). Anti-mouse CD142 antibodies were conjugated with Biotium Mix-n-Stain CF647 (Sigma, MX647S100).

(7) Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signallingBlood cells, molecules & diseasesSeptember 1, 2020Bhagat S, Biswas I, Ahmed R, Khan GA.(SM0241), RiboRuler Low Range RNA Ladder (SM1833), Thermo Fischer (MA, USA); Goat polyclonal TF antibody (AF3178), R&D systems (MN, USA); Mouse monoclonal anti-TF antibody (Ab00516-1.1), Absolute Antibody (CLEV, UK);

(8) SENP3 in monocytes/macrophages up-regulates tissue factor and mediates lipopolysaccharide-induced acute lung injury by enhancing JNK phosphorylationJournal of cellular and molecular medicineMay 1, 2020Chen X, Lao Y, Yi J, Yang J, He S, Chen Y.all purchased from Cell Signaling Technology. Anti‐murine TF antibody was purchased from R&D Systems (AF3178). 2.9 ELISA Bronchoalveolar lavage fluid IL‐6, TNF‐α and IL‐1β levels were assessed using murine ELISA

(9) Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent mannerUpsala journal of medical sciencesAugust 1, 2019Edén D, Panagiotou G, Mokhtari D, Eriksson JW, Åberg M, Siegbahn A.pHSL s563, and HSL from Cell Signaling Technology, USA; TF N1C3 from GeneTex USA; TF AF2339 and TF AF3178 from R&D Systems, USA; IRDye 680CW and IRDye 800CW from LI-COR Biosciences, UK. Human adipocyte isolation Isolation

(10) Protease-activated receptor 2 protects against VEGF inhibitor-induced glomerular endothelial and podocyte injuryScientific reportsFebruary 27, 2019Oe Y, Fushima T, Sato E, Sekimoto A, Kisu K, Sato H, Sugawara J, Ito S, Takahashi N.

(11) Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic diseaseThe Journal of experimental medicineJuly 3, 2017Uderhardt S, Ackermann JA, Fillep T, Hammond VJ, Willeit J, Santer P, Mayr M, Biburger M, Miller M, Zellner KR, et al.baicalein (EMD Millipore) or DMSO control and 40 µg/ml anti–mouse coagulation factor III/TF antibody (AF3178; R&D Systems) or isotype control (normal goat IgG; AB-108-C; R&D Systems) for 1 h at 37°C before stimulation.

(12) Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIbαNature communicationsMay 30, 2017Wang Y, Gao H, Shi C, Erhardt PW, Pavlovsky A, A Soloviev D, Bledzka K, Ustinov V, Zhu L, Qin J, et al.YenZym Antibodies, LLC (South San Francisco, CA). Antibody to TF was purchased from R&D Systems Inc. (AF3178, Minneapolis, MN). Rose Bengal (4, 5, 6, 7-tetrachloro-3′, 6-dihydroxy-2, 4, 5, 7-tetraiodospiro (isobenzofuran-1(3H),

(13) The Myosin II Inhibitor, Blebbistatin, Ameliorates FeCl3-induced Arterial Thrombosis via the GSK3β-NF-κB PathwayInternational journal of biological sciencesJanuary 1, 2017Zhang Y, Li L, Zhao Y, Han H, Hu Y, Liang D, Yu B, Kou J.

(14) Protective and detrimental effects of neuroectodermal cell-derived tissue factor in mouse models of strokeJCI insightJuly 21, 2016Wang S, Reeves B, Sparkenbaugh EM, Russell J, Soltys Z, Zhang H, Faber JE, Key NS, Kirchhofer D, Granger DN, et al.temperature, and incubated overnight at 4°C with a goat anti–mouse TF antibody (1:1,000, R&D Systems, AF3178) or rabbit anti–β-actin (1:1,000, Santa Cruz Biotechnology Inc., sc-47778). After washing, blot was

(15) Hepatocyte tissue factor contributes to the hypercoagulable state in a mouse model of chronic liver injuryJournal of hepatologyJanuary 1, 2016Rautou PE, Tatsumi K, Antoniak S, Owens AP 3rd, Sparkenbaugh E, Holle LA, Wolberg AS, Kopec AK, Pawlinski R, Luyendyk JP, et al.Odyssey blocking buffer (LI-COR Biosciences). Primary antibody against mouse TF (1:500 dilution, clone AF3178, R&D systems) was incubated overnight at 4°C. Primary antibody was detected by fluorescencelabelled

(16) Inflammation drives thrombosis after Salmonella infection via CLEC-2 on plateletsThe Journal of clinical investigationDecember 1, 2015Hitchcock JR, Cook CN, Bobat S, Ross EA, Flores-Langarica A, Lowe KL, Khan M, Dominguez-Medina CC, Lax S, Carvalho-Gaspar M, et al.(AL-21, BD Biosciences), Ly6G (RB6-8C5, eBioscience), CD105 (MJ7/18, BD Biosciences), TF (polyclonal; AF3178, R&D Systems), and podoplanin (eBio8.1.1, eBioscience), as described previously (73). IHC was performed

(17) Regulation of alveolar procoagulant activity and permeability in direct acute lung injury by lung epithelial tissue factorAmerican journal of respiratory cell and molecular biologyNovember 1, 2015Shaver CM, Grove BS, Putz ND, Clune JK, Lawson WE, Carnahan RH, Mackman N, Ware LB, Bastarache JA.counts and differentials were assessed, and there were Microparticle Clot Time (sec) (catalog no. AF3178; R&D Systems, Minneapolis, MN) followed by a biotinylated rabbit anti-goat secondary antibody (Innogenex,

(18) Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated insulin secretion from human pancreatic isletsDiabetologiaNovember 1, 2015Edén D, Siegbahn A, Mokhtari D.Abcam, Cambridge, UK. Mouse TF Ab (clone TF9-10H10) was from AbD Serotec, Kiddlington, UK. Goat TF Ab (no. AF3178) and goat IgG (no. AB-108-C) were from R&D systems. Rabbit TF Ab (no. N1C3) was from GeneTex, Irvine,

(19) Procoagulant and proinflammatory effects of red blood cells on lipopolysaccharide‐stimulated monocytesJournal of Thrombosis and HaemostasisSeptember 1, 2015B. Østerud, D. Unruh, J. O. Olsen, D. Kirchhofer, A. P. Owens, V. Y. Bogdanovwith RBC lysis buffer. Leukocytes were dual-labeled with a goat anti-mouse TF polyclonal antibody (AF3178; R&D Systems, Minneapolis, MN, USA)/Alexa Fluor 594–conjugated rat anti-mouse antibody (Life Technologies,

(20) Tissue factor-targeted lidamycin inhibits growth and metastasis of colon carcinomaOncology lettersSeptember 1, 2013Zhang Q, Liu X, Li C, Liao D, Ouyang Z, Zheng J, Song X.Fluorescence-activated cell sorting (FACS) analysis The cells (1×106) were incubated with a goat anti-mouse TF antibody (AF3178; R&D Systems) in 1 ml PBS for 30 min at room temperature. The cells were washed twice with PBS and incubated

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Mouse Coagulation Factor III/Tissue Factor Antibody

Coagulation Factor III/Tissue Factor Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (23)

Reviews

Coagulation Factor III/Tissue Factor Antibody from R&D Systems, a Bio-Techne Brand

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