CDKN1A/p21 Rabbit Polyclonal Antibody orb11203 from Biorbyt

Product Specs
ItemCDKN1A/p21 Rabbit Polyclonal Antibody
CompanyBiorbyt
Price $250.00Supplier Page
Catalog Numberorb11203
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
ApplicationsFC, ICC, IF, IHC-Fr, IHC-P
Quantity50 ul, 100 ul, 200 ul
TargetCDKN1A
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Research AreaCIP/KIP Family, CIP/KIP family, Cell Cycle, Coactivators and Corepressors, DNA / RNA Binding, DNA and RNA Research, Epigenetics and Nuclear Signaling, Zinc Finger Protein
NCBI Gene AliasesCAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
NCBI Full Gene Namecyclin dependent kinase inhibitor 1A
Concentration1mg/ml
HostRabbit
IsotypeIgG
Molecular Weight18 kDa
ImmunogenKLH conjugated synthetic peptide derived from human P21 (101-164/164aa)
FormatLiquid
ClonalityPolyclonal

Specifications/Features

Application Dilutions: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Questions and Answers

What species does this p21 antibody work with, and has it been used in relevant studies?

The p21 antibody is reactive with mouse, human, and rat species. It has been cited in 17 scientific articles, indicating its utility in various research contexts involving these species.

Can this antibody be used for immunohistochemistry, and what are the recommended dilutions?

Yes, the p21 antibody is suitable for immunohistochemistry (IHC) applications. The recommended dilution for IHC on paraffin-embedded sections is between 1:100 and 1:500.

Is there any information on the applications of this antibody in flow cytometry?

The p21 antibody has been shown to be effective in flow cytometry, with a recommended usage of 1 µg per test. This suggests its potential for analyzing cell populations in various experimental setups.

What is the immunogen used for this p21 antibody, and how might that influence its specificity?

The immunogen for the p21 antibody is a KLH-conjugated synthetic peptide derived from human P21 (amino acids 101-164). This specific immunogen likely contributes to the antibody's specificity for the CDKN1A protein, which is crucial for studies involving cell cycle regulation and cancer research.

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