Fig 1: Photoreceptor connecting cilia are elongated in P15 Bbs8-/- mice.(A) Elongation of GT335 signal in Bbs8-/- mice compared to Bbs8+/+ or Bbs8+/- control littermate. (B) Quantification of the length of GT335 signal in three Bbs8-/- mice and their control littermates. (C) One representative experiment showing the length of GT335 signal in 30 connecting cilia of one Bbs8-/- mouse and its littermate control. (D) Connecting cilia are labeled by anti-glycylated tubulin (TAP952) antibody and the connecting cilia boundaries are demarcated by rootletin (below the connecting cilia) and RP1 (above the connecting cilia). (E) Length of the glycylated tubulin (red) signal shows the elongation of the connecting cilia compartment in Bbs8-/- mice. (F) One representative experiment showing length distribution of 30 connecting cilia in one Bbs8-/- mouse and its littermate control. Statistical significance using two-tailed t-test is shown where * denotes p<0.05, ** denotes p < 0.01, *** denotes p < 0.005, **** denotes p < 0.001, ***** denotes p < 0.0005, ****** denotes p < 0.0001.