Paraffin-embedded human colon carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD160 antibody at 1:200 dilution with overnight incubation at 4 degrees|Paraffin-embedded human colon carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD160 antibody at 1:200 dilution with overnight incubation at 4 degrees|Western blot analysis of rat brain lysates:30ug and human colon carcinoma lysates:30ug using CD160 antibody