Paraffin-embedded human lung carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD25 antibody at 1:200 dilution with overnight incubation at 4 degrees
Supplier Page from Biorbyt for IL2RA/CD25 Rabbit Polyclonal Antibody
Application Dilutions: WB=1:500-2000, Flow-Cyt=0.2ug /test